Accurate, efficient models for advanced disease research.
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Quality: iPSC quality, pluripotency, and cell integrity are maintained through EditCo’s editing process.
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Unparalleled Editing Efficiency: Our automated, optimized platform and superior quality reagents result in high-efficiency editing.
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High Success Rates: EditCo’s robust CRISPR editing process consistently delivers functional gene knockouts.
Experience Unmatched CRISPR Knockout Efficiency in iPSCs
Discover the one-stop solution for reliable and precise knockouts in induced pluripotent stem cells (iPSCs) with EditCo's Engineered Knockout iPS Cells. Our advanced CRISPR editing technology ensures consistently high knockout efficiency (>95%), achieving near-complete functional knockouts even before clonal selection. Utilizing high-quality synthetic non-integrating reagents, our proprietary automated process maintains cell viability and pluripotency, ensuring your cells are artifact-free and your experimental integrity remains intact. Choose EditCo’s Engineered Knockout iPS Cells, available as 100% knockout guaranteed clones or as efficient, non-sorted pools, to elevate your gene function and disease linkage studies. Unlock unprecedented potential and accelerate your research with EditCo.
EditCo’s Automated iPS Cell Line Editing Process
Guaranteed iPSC CRISPR Knockout, Ready for Downstream Applications
Features
Cell Source
- EditCo supplied (standard)
- Customer supplied
Available Edits
- Indel (standard) or fragment deletion
- Homozygous (standard) or heterozygous edits
CRISPR Design
- Synthetic modified sgRNA (standard)
- Multiple synthetic modified sgRNAs
Add-Ons
- Additional clones
- QC: Pluripotency Testing
- QC: Karyotype Testing
Deliverables
- Regular updates on your order's progress
- 2 independent clones with the required knockout (2 vials of each clone with 5x10⁵ cells/vial)
- Mock-transfected cell pools (2 vials with 5x10⁵ cells/vial)
- Sequence of synthetic sgRNA used
- Primer sequences used for NGS sequencing
- NGS sequencing analysis report for each edited pool after expansion.
- Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC
Sequencing deliverable note: For large fragment deletions and non-human/mouse cell types, an alignment between the Sanger sequencing data of the edited clone and the reference will be provided
Pre-clonal iPSC CRISPR Knockout Cells: Your Unique Assay Validation Tool
EditCo’s Knockout iPS Cell Pools offer a heterogeneous population of induced pluripotent stem cells. Each pool contains iPSCs edited to knock out your gene of interest through CRISPR transfection and a population of unedited cells. Delivered prior to clonal isolation, these pools combine the benefits of EditCo’s optimized CRISPR editing process with high knockout efficiency. For a homogeneous cell population ready for immediate use, explore our Knockout iPS Cell Clones with automated clonal isolation.
Features
Cell Source
- EditCo supplied (standard)
- Customer supplied
Available Edits
- Indel (standard) or fragment deletion
- A heterogeneous population of edited and unedited cells
CRISPR Design
- Synthetic modified sgRNA (standard)
- Multiple synthetic modified sgRNAs
Add-Ons
- QC: Pluripotency testing
Deliverables
- Regular updates on your order's progress
- Edited cell pools (2 vials with 5x10⁵ cells/vial)
- Mock-transfected cell pools (2 vials with 5x10⁵ cells/vial)
- Sequence of synthetic sgRNA used
- Primer sequences used for NGS sequencing
- NGS sequencing analysis report for each edited pool after expansion.
- Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC
Use Our iPS Cell Lines or Onboard Your Own
EditCo-supplied cell lines available for all engineered iPS cell orders at no additional cost
* Parental vials available for evaluation prior to booking an edit
Unprecedented Knockout Editing Efficiency
EditCo’s Knockout iPS Cells deliver reliable protein knockout enabling your gene function studies.
Figure 1. Modified gRNAs were transfected along with Cas9 as RNPs to knock out RELA in four individual iPS cell lines. Each population of cells was PCR-amplified around the cut site, Sanger-sequenced, and submitted for ICE analysis to determine editing efficiency.
EditCo Delivers Pluripotent Edited Cells
EditCo’s CRISPR Knockout Cell Clones eliminate the time and hassle of doing CRISPR editing and creating clonal cell lines so you can focus on your research.
Figure 2. iPS cells were assessed for standard pluripotency markers, three days post-editing.
Validate your Assays with up to 95% Protein Knockout
EditCo’s Knockout Cell Pool gives you the protein knockout you need in the cell line you want, enabling you to either assay pools directly or quickly move to isolate single cell clones.
Figure 3. EditCo’s Knockout Cell Pool achieved 95% protein knockout without clonal expansion. A cell pool with a surface protein knocked out was generated in U2OS cells with a Knockout Score of 87% (left panel, which is the percentage of indels that either introduce a frameshift mutation or are larger than 21 nucleotides, analyzed by ICE). Levels of this protein were analyzed by flow cytometry (blue) and were reduced by 95% relative to wild type (green, right panel). Negative control samples were stained with an isotype control antibody (purple). Data provided by Eurofins-DiscoverX.
Resources
Unlock advanced CRISPR knock-ins for neuroscience and regenerative medicine research.
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Quality: Maintain iPSC quality, pluripotency, and cell integrity with EditCo’s editing process.
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Precision: Focus on the phenotype of your desired edit and minimize off-target effects with transient transfection.
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Unlocked Capacity: Experience faster lead times with EditCo’s high-throughput automated platform.
Achieve Precise CRISPR Knock-Ins While Preserving iPSC Quality
Elevate your neuroscience, cardiovascular, or regenerative medicine research with EditCo's precise CRISPR knock-in edits in iPSCs. Let EditCo handle the complex editing process so you can focus on assay development, differentiation, and other downstream applications. Our robust, automated platform ensures high editing efficiencies while maintaining cell quality and pluripotency, providing you with reliable and artifact-free CRISPR-edited iPSCs.
EditCo offers a range of knock-ins, including single nucleotide variants (SNVs), tags, and <100 bp insertions, available in both homozygous and heterozygous states, and in clone or pool formats. Empower your research and accelerate your discoveries with EditCo's advanced CRISPR technology.
EditCo’s Automated iPS Cell Line Editing Process
Move Directly Into Your Functional Assays with Confidence
Features
Cell Source
- EditCo supplied (standard)
- Customer supplied
Genetic Modifications
- SNV, Tag, or Insertion
- Homozygous or Heterozygous Edits
CRISPR Design
- Synthetic modified sgRNA (standard)
- Donor ssODN (Standard)
Add-Ons
- Additional clones
- QC: Pluripotency Testing
- QC: Karyotype Testing
Deliverables
- Regular milestone updates on your order's progress
- 2 independent clones with the required knock-in (2 vials of each clone with 500,000 cells/vial)
- Mock-transfected cell pools (2 vials with 500,000 cells/vial)
- Sequence of synthetic sgRNA and HDR template used
- Primer sequences used for NGS sequencing
- NGS sequencing analysis report for each edited pool after expansion.
- Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC
Sequencing deliverable note: For large knock-ins and non-human/mouse cell types an alignment between the Sanger sequencing data of the edited pool and the knock-in sequence will be provided
High Efficiency CRISPR Knock-ins with DIY Clonal Isolation
EditCo’s Knock-in iPS Cell Pools provide a heterogeneous mix of CRISPR-edited and unedited cells, giving you high editing efficiency without clonal isolation. Benefit from EditCo’s optimized CRISPR platform, achieving precise edits while allowing you the flexibility to perform clonal isolation yourself. For those seeking a fully automated process, explore our Knock-in iPS Cell Clones for guaranteed homogeneity and convenience.
Features
Cell Source
- EditCo supplied (standard)
- Customer supplied
Available Edits
- SNV, Tag, or Insersion
- A heterogeneous population of edited and unedited cells
CRISPR Design
- Synthetic modified sgRNA (standard)
- Donor ssODN (standard)
Add-ons
- QC: Pluripotency testing
Deliverables
- Regular updates on your order's progress
- Edited cell pools (2 vials with 5 million cells/vial)
- Mock-transfected cell pools (2 vials with 5 million cells/vial)
- Sequence of synthetic sgRNA used
- Primer sequences used for NGS sequencing
- NGS sequencing analysis report for each edited pool after expansion.
- Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC
Sequencing deliverable note: For large knock-ins and non-human/mouse cell types an alignment between the Sanger sequencing data of the edited pool and the knock-in sequence will be provided
Use Our iPS Cell Lines or Onboard Your Own
EditCo-supplied cell lines available for all engineered iPS cell orders at no additional cost
* Parental vials available for evaluation prior to booking an edit
Precision Knock-in Clones and Pools
Robust Editing Across Different iPSC Lines
EditCo’s robust, automated editing platform results in high knock-in efficiency across a range of workhorse iPSC lines and patient derived iPS cell lines, ensuring a high success rate in any iPSC line.
Figure 1. Average knock-in editing efficiency by knock-in type. Knock-in efficiencies were all above 31% and performed in a variety of EditCo-supplied and customer supplied iPSC lines. Tags and Small KIs were all less than 100 bps in length. Knock-in edits were performed using RNPs and ssODNs and editing efficiency was assessed in the pool stage before proceeding to clonal isolation. DNA in each population of cells was PCR-amplified around the cut site, Sanger-sequenced, and submitted for ICE analysis to quantify editing efficiency.
100% SNV Editing in iPS Cell Clones
Focus on your research by letting EditCo deliver your precise SNV knock-ins enabling creation of isogenic lines.
Figure 2. A CRISPR-edited homozygous clone containing a single nucleotide change (top) relative to the wild type control (bottom). An orange box encloses the single nucleotide change from cytosine (C) to thymine (T). The target sequence is underlined in white and the PAM site is underlined with a red dashed line in the control trace. SNV knock-ins are also available as pools.
EditCo Delivers Pluripotent Edited Cells
Quality comes first in the development of EditCo’s Knock-in iPS Cells by using our automated workflow and transient RNPs to maintain pluripotency of your cells.
Figure 3. iPS cells were assessed for standard pluripotency markers, three days post-editing.
Resources
Integrating our core CRISPR expertise, high-quality reagents, and automated processes, we deliver the best edited cell-based models at any scale.
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