Poster: Characterisation of TREM2 R47H iPSC-Derived Microglia, & Assessmentof Their Suitability for NLRP3 Inflammasome Drug Discovery

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By integrating EditCo’s CRISPR-based gene engineering, BrainXell’s specialized iPSC-derived neural cell solutions, and Arctoris’s advanced screening capabilities, we developed TREM2 R47H iPSC-derived microglia for a deeper look at the NLRP3 inflammasome. Early results provided key findings that open the door for new advances in neurodegeneration research.

Conclusions

• CRISPR edited, iPSC-derived human TREM2 R47H microglia show minor morphological and marker expression profiles, and reduced phagocytic activity compared with WT microglia
• TREM2 R47H microglia are hypersensitive to inflammatory stimuli in both general inflammation and chemokine secretion, and NLRP3 inflammasome activation
• Human iPSC-derived microglia are amenable to automated culture protocols for screening