Intelligently Designed Multi-guide RNA Improves CRISPR Knockout Efficiency with Single Exon Fragment Deletions

Learn how EditCo's intelligent multi-gRNA design is game-changing CRISPR

How do you create a novel CRISPR design method that dramatically increases knockout efficiency?

The EditCo team has used our learnings from over 300,000 CRISPR edits to refine our intelligent multi-guide CRISPR design to improve your CRISPR editing. 

Our application note delves into the power of CRISPR-Cas9 in gene knockouts, highlighting EditCo's innovative multi-guide approach. With higher knockout efficiency and complete protein-level knockouts, this method is set to accelerate your functional genomics and disease modeling projects.

Key findings

• EditCo’s multi-guide sgRNA uses 3 sgRNAs targeted to a single exon of the gene of interest. 
• Generating 21+ bp deletions in targeted regions results in a higher knockout efficiency than any individual guide alone.
• Complete protein-level knockouts were consistently observed in non-clonal cell populations after editing by multi-gRNA. 

Ready to elevate your research? Download our application note today and explore how EditCo’s multi-guide gene editing can transform your genetic studies!