Accurate, efficient models for advanced disease research.
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Quality: iPSC quality, pluripotency, and cell integrity are maintained through EditCo’s editing process.
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Unparalleled Editing Efficiency: Our automated, optimized platform and superior quality reagents result in high-efficiency editing.
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High Success Rates: EditCo’s robust CRISPR editing process consistently delivers functional gene knockouts.
Experience Unmatched CRISPR Knockout Efficiency in iPSCs
Discover the one-stop solution for reliable and precise knockouts in induced pluripotent stem cells (iPSCs) with EditCo's Engineered Knockout iPS Cells. Our advanced CRISPR editing technology ensures consistently high knockout efficiency (>95%), achieving near-complete functional knockouts even before clonal selection. Utilizing high-quality synthetic non-integrating reagents, our proprietary automated process maintains cell viability and pluripotency, ensuring your cells are artifact-free and your experimental integrity remains intact. Choose EditCo’s Engineered Knockout iPS Cells, available as 100% knockout guaranteed clones or as efficient, non-sorted pools, to elevate your gene function and disease linkage studies. Unlock unprecedented potential and accelerate your research with EditCo.
EditCo’s Automated iPS Cell Line Editing Process
Single-guide RNA Knockout Cell Pools
Pre-clonal iPSC CRISPR KO Cells: Your Unique Assay Validation Tool
EditCo’s Knockout iPS Cell Pools offer a heterogeneous population of induced pluripotent stem cells. Each pool contains iPSCs edited to knock out your gene of interest through CRISPR transfection and a population of unedited cells. Delivered prior to clonal isolation, these pools combine the benefits of EditCo’s optimized CRISPR editing process with high knockout efficiency. For a homogeneous cell population ready for immediate use, explore our Knockout iPS Cell Clones with automated clonal isolation.
Features
Cell Source
- EditCo supplied (standard)
- Customer supplied
Available Edits
- Indel (standard) or fragment deletion
- A heterogeneous population of edited and unedited cells
CRISPR Design
- Synthetic modified sgRNA (standard)
- Multiple synthetic modified sgRNAs
Add-Ons
- QC: Pluripotency testing
Deliverables
- Regular updates on your order's progress
- Edited cell pools (2 vials with 5x10⁵ cells/vial)
- Mock-transfected cell pools (2 vials with 5x10⁵ cells/vial)
- Sequence of synthetic sgRNA used
- Primer sequences used for NGS sequencing
- NGS sequencing analysis report for each edited pool after expansion.
- Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC
Unprecedented Knockout Editing Efficiency
EditCo’s Knockout iPS Cells deliver reliable protein knockout enabling your gene function studies.
Figure 1. Modified gRNAs were transfected along with Cas9 as RNPs to knock out RELA in four individual iPS cell lines. Each population of cells was PCR-amplified around the cut site, Sanger-sequenced, and submitted for ICE analysis to determine editing efficiency.
Validate your Assays with up to 95% Protein Knockout
EditCo’s Knockout Cell Pool gives you the protein knockout you need in the cell line you want, enabling you to either assay pools directly or quickly move to isolate single cell clones.
Figure 2. EditCo’s Knockout Cell Pool achieved 95% protein knockout without clonal expansion. A cell pool with a surface protein knocked out was generated in U2OS cells with a Knockout Score of 87% (left panel, which is the percentage of indels that either introduce a frameshift mutation or are larger than 21 nucleotides, analyzed by ICE). Levels of this protein were analyzed by flow cytometry (blue) and were reduced by 95% relative to wild type (green, right panel). Negative control samples were stained with an isotype control antibody (purple). Data provided by Eurofins-DiscoverX.
Single-guide RNA Knockout Cell Clones
Guaranteed iPSC CRISPR Knockout, Ready for Downstream Applications
EditCo’s Knockout iPS Cell Clones offer a homogeneous population of induced pluripotent stem cells with your desired protein functionally knocked out, guaranteed. These clones are the simplest way to harness the power of CRISPR in your lab, enabling you to focus on your research and downstream applications with confidence.
Features
Cell Source
- EditCo supplied (standard)
- Customer supplied
Available Edits
- Indel (standard) or fragment deletion
- Homozygous (standard) or heterozygous edits
CRISPR Design
- Synthetic modified sgRNA (standard)
- Multiple synthetic modified sgRNAs
Add-Ons
- Additional clones
- QC: Pluripotency Testing
- QC: Karyotype Testing
Deliverables
- Regular updates on your order's progress
- 2 independent clones with the required knockout (2 vials of each clone with 5x10⁵ cells/vial)
- Mock-transfected cell pools (2 vials with 5x10⁵ cells/vial)
- Sequence of synthetic sgRNA used
- Primer sequences used for NGS sequencing
- NGS sequencing analysis report for each edited pool after expansion.
- Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC
Sequencing deliverable note: For large fragment deletions and non-human/mouse cell types, an alignment between the Sanger sequencing data of the edited clone and the reference will be provided
EditCo Delivers Pluripotent Edited Cell Clones
EditCo’s CRISPR Knockout Cell Clones eliminate the time and hassle of doing CRISPR editing and creating clonal cell lines so you can focus on your research.
Figure 3. iPS cells were assessed for standard pluripotency markers, three days post-editing.
Use Our iPS Cell Lines or Onboard Your Own
EditCo-supplied cell lines available for all engineered iPS cell orders at no additional cost
* Parental vials available for evaluation prior to booking an edit
Resources
Integrating our core CRISPR expertise, high-quality reagents, and automated processes, we deliver the best edited cell-based models at any scale.
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