Your cell lines, our confident knockouts
Reliable Editing: >80% KO editing efficiency with EditCo’s XDel guide design
Rapid Results: Edited cells in 4 weeks or faster from cell line receipt
High-throughput: Streamline screening with our fibroblast editing at library scales
Fit to Your Needs: A flexible CRISPR platform enables efficient editing of customer-supplied cells
Primary Fibroblast KO Pools are made-to-order knockouts with editing efficiencies >80% generated in 4 weeks or faster from receiving IDEXX approved cells. EditCo consistently achieves >90% editing efficiency, creating cell pools that are functionally similar to a clone. As primary cell clones are biologically difficult to produce, EditCo’s high editing efficiency provides a unique approach to generating cell populations of interest.
Whether you are leveraging CRISPR for validating targets for drug discovery or modeling a biological process for the investigation of a novel gene of interest, failed experiments lead to a loss of time and resources. EditCo’s Primary Fibroblast KO Pools are the solution for obtaining quick and reliable functional data while reducing the risk for false negatives in your critical assays.
What We Provide
EditCo’s proprietary editing protocols deliver high-efficiency edits at scale. Our Knockout Fibroblast Cells, powered by XDel Technology, provide researchers with the confidence and reproducibility they need for guaranteed CRISPR knockouts.
Example editing efficiency of a large library screen: 90% of targets with greater than 80% editing efficiency across 64 total targets in human large intestine primary fibroblasts.
Edited Fibroblast Cell Options
Single-edit Knockout Cell Pools
- 2 vials of 1M edited cell pools (1M/vial)
- 2 vials of control mock transfected cell pools
- Sequence of synthetic sgRNAs used
- Primer sequences used for PCR and NGS sequencing
- NGS sequencing analysis reports
- Comprehensive QC report including Mycoplasma tests and passage number
Knockout Cell Screening Libraries
- 2 vials of each pool with 20k cells per vial in arrayed format
- 5 control pools per rack: 2 mock WT, 2 multi-guide KO, 1 single-guide KO
- Sequence of synthetic sgRNAs used
- Primer sequences used for PCR and Sanger sequencing
- Sanger sequencing analysis reports
- Comprehensive QC report including Mycoplasma tests and passage number
For Additional Information on our Primary Fibroblast offering, download our flyer below.
Downstream Applications and Partnerships
EditCo’s made-to-order knockout fibroblast services enable you to further research in CNS disorders, wound healing, cystic fibrosis, autoimmunity, cancer, or other disease areas where fibroblasts have been implicated. Furthermore, EditCo’s extensive Discovery Partners Ecosystem creates a seamless workflow from EditCo’s CRISPR engineered cells to phenotypic data, providing researchers with a faster, more efficient, and more reliable way to drive their discoveries forward.
Resources
Integrating our core CRISPR expertise, high-quality reagents, and automated processes, we deliver the best edited cell-based models at any scale.
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