Precision CRISPR Knock-ins for Streamlined and Efficient Success
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High success rate: Our automated optimization conquers cell line variability, identifying the perfect transfection conditions for maximum editing efficiency.
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Minimize off-targets: With stringent guide design criteria and RNP-based delivery, EditCo ensures precise edits with minimal off-target effects.
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Accessibility: Expand your research without overspending. Model more genes or variants in more cell lines and see how much farther your budget can go.
Access CRISPR Cell Lines Without Sacrificing Time
EditCo’s Knock-in Immortalized Cells are your quickest path to integrating engineered cells into your research. Whether modeling disease with SNVs or tagging proteins for in-depth studies, DIY CRISPR projects often come with unforeseen challenges and delays, typically requiring several experimental repeats and adding months to your timeline. EditCo’s advanced automated transfection optimization and top-tier reagents maximize editing efficiency, guaranteeing a successful CRISPR edit.
Unlock your research potential with EditCo’s Knock-in Immortalized Cells and accelerate your path to discovery.
EditCo’s Automated Immortalized Cell Line Editing Process
Single-guide RNA Knock-in Cell Pools
Economical High-throughput CRISPR Optimization for Knock-in Immortalized Cells
EditCo’s Knock-in Immortalized Cell Pools provide a cost-effective solution, offering a mixed population of transfected cells with both edited and unedited cells. Harness the power of EditCo’s high-throughput CRISPR optimization and advanced knock-in strategies while managing clonal isolation on your terms. For those looking to save hands-on time and effort, explore our Knock-in Immortalized Cell Clones, where we handle the isolation process for you.
Features
Cell Source
- EditCo supplied (standard)
- Customer supplied
Available Edits
- SNV
- Tag
- Large insertion
CRISPR Design
- Synthetic modified sgRNA (standard)
- DNA HDR template (standard)
Deliverables
- Periodic updates on your order's progress
- Mock-transfected cell pools (2 vials)
- Sequence of synthetic sgRNA and HDR template used
- Primer sequences used for NGS sequencing
- NGS sequencing analysis report for each edited pool after expansion.
- Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC
Sequencing deliverable note: For large knock-ins and non-human/mouse cell types an alignment between the Sanger sequencing data of the edited pool and the knock-in sequence will be provided
Successful Knock-ins in Any Cell Line
EditCo’s CRISPR Knockout Cell Clones eliminate the time and hassle of doing CRISPR editing and creating clonal cell lines so you can focus on your research.
Figure 1. Successful knock-in editing, even in hard-to-edit cell lines, enabled by our automated transfection optimization protocol. gRNAs and ssODNs were designed to induce a 33nt knock-in and then transfected into the 9 different immortalized cell lines indicated. Post recovery, knock-in editing efficiencies were measured in the mixed population of edited and unedited cells using EditCo’s ICE Knock-in analysis tool. The cells are then either used directly for assays or isolated into clonal populations.
Figure 2. High knock-in efficiencies across numerous loci. sgRNA and ssODNs were designed to induce a 9-nt knock-in at numerous different loci, then transfected into multiple different cell types. Post cell recovery, knock-in editing efficiencies were assessed using EditCo’s ICE Knock-in analysis tool. The values displayed above represent the percentage of each total population of cells that have the desired knock-in edit present.
Single-guide RNA Knock-in Cell Clones
Precise Knock-in Immortalized Cell Clones Done for You
EditCo’s Knock-in Immortalized Cell Clones offer a clonal population of cells with sequence-verified knock-ins. Whether you need to endogenously tag a protein or alter an SNV, our Knock-in Immortalized Clones provide the simplest path to achieving precise, high-quality edits in your lab. Unlike our pool format, these clones undergo EditCo’s automated isolation process, ensuring you receive a population derived from a single cell with your desired knock-in.
Features
Cell Source
- EditCo supplied (standard)
- Customer supplied
Genetic Modifications
- SNV
- Tag
- Large insertion
CRISPR Design
- Synthetic modified sgRNA (standard)
- DNA HDR template (standard)
Add-Ons
- Additional clones
- Additional vials
- Intermediate pools
Deliverables
- Periodic milestone updates as we make progress on your order
- 2 individual edited clones (2 vials of each clone)
- Mock-transfected cell pools (2 vials)
- Sequence of synthetic sgRNA and HDR template used
- Primer sequences used for NGS sequencing
- NGS sequencing analysis report for each edited pool after expansion.
- Comprehensive QC report that includes the following information: mycoplasma test (positive/negative), passage number, and analysis for add-on QC
Sequencing deliverable note: For large knock-ins and non-human/mouse cell types an alignment between the Sanger sequencing data of the edited pool and the knock-in sequence will be provided
Resources
Integrating our core CRISPR expertise, high-quality reagents, and automated processes, we deliver the best edited cell-based models at any scale.
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