CRISPR Kit Reagents: CRISPR Transfection Reagents, Cas9 Nucleases, gRNA Control Kits and more

High quality SpCas9 nuclease available as a standalone product or as an add-on to your Gene Knockout Kit or gRNA Libraries. 

What's included

• Sizes: 300 pmol, 1000 pmol
• Concentration: 20 µM (20 pmol/µl)
• Format: Individual tubes

This multi-guide positive control is a validated trio of gRNA targeting TRAC in human cell types. Use this control to determine optimal conditions for maximum editing efficiency in your multi-guide experiments involving our Arrayed CRISPR Libraries or Gene Knockout Kit. For the full kit of the positive control with the addition of sequencing and PCR primers as well as Cas9 nuclease, purchase the Transfection Optimization Kit.

What's included

• 1.5 nmol of chemically modified synthetic multi-guide sgRNA

Positive Control sgRNA (mod), Human CDC42BPB

This positive control is a validated gRNA sequence targeting CDC42BPB with editing efficiency of 80% - 95% in multiple cell types. Use this control to determine optimal conditions for maximum editing efficiency in your cell models.

What's included

• 1 nmol of chemically modified synthetic sgRNA

Positive Control sgRNA (mod), Human RELA

This positive control is a validated gRNA sequence targeting CDC42BPB with editing efficiency of 80% - 95% in multiple cell types. Use this control to determine optimal conditions for maximum editing efficiency in your cell models.

What's included

• 1 nmol of chemically modified synthetic sgRNA

Positive Control sgRNA (mod), Mouse Rosa26

A single guide RNA targeting Rosa26 that you can use to optimize transfection conditions in your cell line to achieve maximum editing efficiency. It is important to note that the mouse Positive Control contains only 1 sgRNA compared to your Mouse Gene Knockout Kit which contains between 1-3 sgRNAs. The Mouse Positive Control can assist in selecting the optimal CRISPR transfection conditions.

What's included

• 1 nmol of chemically modified synthetic sgRNA

Negative Control sgRNA (mod) #1

Non-targeting gRNA sequences are designed to not target any genomic location in the human & mouse species. This control generates a non-edited control when analyzing edited cells.

What's included

• 1 nmol of chemically modified synthetic sgRNA

Negative Control sgRNA (mod) #2

Non-targeting gRNA sequences are designed to not target any genomic location in the human & mouse species. This control generates a non-edited control when analyzing edited cells.

What's included

• 1 nmol of chemically modified synthetic sgRNA

A simplified easy-to-use kit designed for users to optimize their transfection protocols and become familiar with EditCo's chemically modified sgRNAs and CRISPR/Cas9 technology.

What's included

• 2x Positive Control sgRNA (mod), Human RELA & CDC42BPB (1 nmol each)
• Negative Control sgRNA (mod) #1 (1 nmol)
• SpCas9 2NLS Nuclease (300 pmol)
• PCR Primer Mix (RELA & CDC42BPB) (20 µl each)
• Sequencing Primer (RELA & CDC42BPB) (20 µl each)
• Nuclease-free Water & TE Buffer (1.5 ml each)

This kit contains a positive control multi-guide sgRNA that can be used to optimize transfection parameters prior to knocking out your gene of interest. The positive control multi-guide sgRNA is composed of three guides that induce a fragment deletion in the human TRAC gene. This kit is meant to be used in conjunction with the Gene Knockout Kit, which also uses a multi-guide strategy.

What's included

• 1x Positive Control Multi-guide sgRNA (mod), Human TRAC
• SpCas9 2NLS Nuclease (300 pmol)
• PCR Primer Mix (20 µl)
• Sequencing Primer (20 µl)
• Nuclease-free Water & TE Buffer (1.5 ml each)

10 mM Tris, 1 mM EDTA, pH 8.0. For resuspending or diluting gRNA libraries.

What's included

• 1.5 nmol of chemically modified synthetic multi-guide sgRNA

Nuclease-free Water

For resuspending or diluting gRNA libraries.

What's included

• 5 tubes, 1.5ml each